Abstract: OBJECTIVE: To explore the value of Zona-free Hamster Oocyte Sperm Penetration Assay (SPA) and DNA integrity monitoring in in vitro fertilization-embryo transfer (IVF-ET). METHODS: 41 men from infertile couples opting for IVF-ET were divided into two groups of pure male sterility or female infertility depending on the cause of infertility.They were assessed by comparing male ages,fertility index,rates of penetration,DNA fragmentation,IVF fertility, ovum cleavage,good quality embryo,implantation and clinical pregnancy. RESULTS: Two groups showed significant differences in penetration rates,fertility index,DNA fragment rates(P<0.05)，but no differences in male ages,the rates of IVF fertility, ovum cleavage,good quality embryo, implantation and clinical pregnancy(P>0.05). In groups of male sterility and female infertility,obvious correlations were observed in penetration rates with the rates of IVF fertility,ovum cleavage and good quality embryo,and DNA fragment rates with the rates of IVF fertility, ovum cleavage and good quality embryo, respectively(P<0.05). CONCLUSION: SPA and DNA fragmentation monitoring may be valuable in assisted reproductive technology.

Key words: assisted reproductive technology, semen, zona-free hamster oocyte sperm penetration assay, sperm DNA, in vitro fertilization-embryo transfer