Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (6): 443-446.doi: 10.3969/j.issn.1004-616x.2009.06.009

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Changes of Immunological Function after Two Separate Injections of Cyclophosphamidec in SD Rats

WU You-ping1; ZHANG Sheng2; CHEN Yan-hong1;WU Hong-hai1;YU Ya-nan1   

  1. 1.The Center for Drug Safety Evaluation and Research of Zhejiang University,Hangzhou 310058; 2.National Safety Evaluation for New Drug, Zhejiang Academy of Medical Sciences, Hangzhou 310013,Zhejiang, China
  • Received:2009-01-14 Revised:2009-03-31 Online:2009-11-30 Published:2009-11-30
  • Contact: WU You-ping

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Abstract: BACKGROUND AND AIM: To explore the possible mechanism that two administrations of cyclophosphamide (CP) could potentiate immunity in normal SD rats. MATERIALS AND METHODS: Peripheral blood was analyzed by ADVIA 2120 blood-counter system and flow cytometry after two treatments with 60 mg/kg of CP in vivo. Spleen lymphocyte proliferation was measured with Mono-nuclear cell direct cytotoxicity assay (MTT), the anti-OVA antibody response were assessed by ELISA. RESULTS: Cells in peripheral blood were decreased six days after the first CP administration, but increased nine days after second CP injection. A significant elevation was also observed in splenic proliferation. Consistently, the ratio of CD4＋ T lymphocytes and CD4＋/CD8＋ was markedly increased (P<0.05). However,anti-OVA antibody response was suppressed during these processes. CONCLUSION:These changes might be associated with the immune potentiating effect of CP, treated with two CP (60 mg/kg) injections in 6 days might enhance the immunological function of SD rats.

Key words: cyclophosphamide, hematological index, lymphocyte subset

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