Abstract: BACKGROUND AND AIM: To examine the significance of P504S, p63, 34βE12 cocktail in the differential diagnosis of prostate lesion in needle biopsy specimen. MATERIALS AND METHODS: The expressions of P504S,P63 and 34βE12 were examined in the biopsy specimens of 32 prostate cancer(PCa),16 prostatic atypical adenomatous hyperplasia (AAH)and 44 benign prostatic hyperplasia (BPH) using immunohistochemical method with triple-antibody cocktail (P504S, P63, 34βE12)staining and double-color chromogens in single paraffin sections. RESULTS: PCa stained positive for P504S and negative for P63 and 34βE12, and the positivity rate of P504S was 100％. The positive rates in AAH were all 75％ for the three proteins. BPH strongly expressed P63 and 34βE12, but not P540S. The expressions of P504S, P63 and 34βE12 were significantly different among PCa, AAH and BPH (P<0.05). CONCLUSION: Cocktail staining may be an effective approach in the diagnosis and differential diagnosis of prostate lesion.P504S was a sensitive and specific marker for PCa. P63 and 34βE12 cocktail staining could increase the sensitivity and specificity for the basal cell layer. The triple-antibody cocktail staining could improve diagnostic accuracy of prostate lesion in needle biopsy specimen, and important for the early diagnosis of prostate cancer.

Key words: prostate cancer, P504S, P63, 34βE12, immunohistochemistry