Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (5): 334-338.doi: 10.3969/j.issn.1004-616x.2009.05.002

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Nerve Growth Factor on the Proliferation of Human Pancreatic Carcinoma Cells

MENG Ling-xin1;DING Zhao-jun1; CHEN Xi-ping1;ZHANG Hong1;CHI Yu-hua1; ZHAO Zhi-gang2;SHI Yu-rong2   

  1. 1.Oncology Department,Jining Medical College Affilated Rizhao People＇s Hospital, Rizhao Cancer Therapy Center,Rizhao 276826,Shandong;2. 211-Projects State Key Laboratory of Experimental Cancer,Tianjin Medical University Cancer Hospital,Tianjin 300060,China
  • Received:2009-03-19 Revised:2009-04-09 Online:2009-09-30 Published:2009-09-30
  • Contact: MENG Ling-xin

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Abstract: BACKGROUND AND AIM: To investigate the effect of nerve growth factor(β-NGF) on the proliferative ability and cell cycle of human pancreatic carcinoma cells MIA PaCa-2. MATERIALS AND METHODS: Colony forming efficiency of MIA PaCa-2 cells treated with β-NGF was higher than normal group(P<0.05),especially with the 100 ng/ml β-NGF group.Absorbence gradually increased,along with β-NGF concentration enhancement and culture time prolongation.Proliferation rate reached 48.68％ when cultured 72 hours in 100 ng/ml β-NGF group.However, absorbence gradually decreased in K252a group at 24,48,72 h and 96 h.Inhibition rate was reduced to 18.05％ in 100 ng/ml K252a group.Effect of different concentrations of β-NGF and K252a on MIA PaCa-2 cells proliferation was evaluated by MTT and Flow Cytometry. RESULTS: β-NGF promoted MIA PaCa-2 proliferation and growth which were markedly inhibited by K252a(P<0.05). CONCLUSION: β-NGF could enhance the proliferation of MIA PaCa-2 pancreatic carcinoma cells.

Key words: pancreatic carcinoma, nerve growth factor(β-NGF), cell culture, flow cytometry, proliferation

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