Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (4): 298-301.doi: 10.3969/j.issn.1004-616x.2009.04.012

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Effects of Polychlorinated Biphenyl on Hippocampal Neuron Cells in Rats

ZHAO Hong-bin1; ZHOU Huan-fa1; MA Hui1; LI Fu-jun1;YANG Yin-shu2; WANG Xian-hua1   

  1. 1.Department of Animal Laboratory, Lanzhou General Hospital; Lanzhou Command; PLA; Lanzhou 730050;2. Lanzhou Command Center for Disease Prevention and Control;Lanzhou 730020;Gansu;China
  • Received:2008-10-27 Revised:2008-12-02 Online:2009-07-30 Published:2009-07-30
  • Contact: ZHAO Hong-bin

Abstract: BACKGROUND AND AIM: To study the effects of polychlorinated biphenyl(PCB) on hippocampal neuron cells in rats. MATERIALS AND METHODS: Bilateral hippocampi were isolated from neonatal Wistar rats; cellular suspensions were prepared under sterile conditions. Rats were divided into control group and three experimental groups; the dosage of PCB were low (1×10-8 mol/L ); middle(1×10-7 mol/L) and high (1×10-6 mol/L);respectively. Light microscope and immunohistochemical technology were used to examine the effects of PCB on structure and function of hippocampal neuron cells in rats. RESULTS: The structural damage of hippocampal neuron cells was related to the dose of PCB; neuron cells were most seriously affected in the high dose group. Axons and dendrites disappeared to remnants; with atrophy of cell bodies. PCB could induce bcl-2 and TGF-β1 expressions in hippocampal neuron cells; bcl-2 expression was detected in control; low dose and middle dose groups; strongest in the low dose group; while bcl-2 expression was not found in the high dose group. TGF-β1 expression was positive in control group. PCB could inhibit TGF-β1 expression in low dose group ; but the expression was up-regulated by PCB in middle dose group. CONCLUSION: PCB could affect the development of hippocampal neuron cells.

Key words: polychlorinated biphenyl(PCB), hippocampal neuron cells, bcl-2, TGF-β1

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