Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (2): 123-126.doi: 10.3969/j.issn.1004-616x.2009.02.011

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Pilot Study of Apoptosis and Mechanism of K562 Cells Exposed to Cerium Chloride and Cerium Chloride Combined with Gamma Radiation

HAO Shu-xia,WANG Chun-yan,LU Hui-min   

  1. National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
  • Received:2008-06-18 Revised:2008-09-10 Online:2009-03-30 Published:2009-03-30

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Abstract: K562； 凋亡； Bcl-2/Bax； 细胞周期BACKGROUND AND AIM: To investigate the apoptotic effect and mechanism of K562 cells exposed to cerium chloride(CeCl3) and CeCl3 combined with gamma radiation. MATERIALS AND METHODS: Apoptosis and cell cycle of K562 cells were examined using flow cytometry and expressions of Bcl-2 and Bax were assessed with Western blot. RESULTS: Compared with control,CeCl3 exposure for 26 h ,gamma irradiation,and both combined could induce apoptosis of K562 cells(P<0.05).The cells in S phase were reduced in CeCl3 group(P<0.05), in irradiation and combined treatment group also(P<0.01). The cell cycle was blocked in G2/M phase in irradiation group and combined treatment group compared with control. The Bcl-2/Bax ratio in all 3 groups were lower than control. CONCLUSION: CeCl3 and CeCl3 combined with gamma radiation induced K562 cells apoptosis via mitochondrial-mediated apoptosis pathway. The apoptosis of the combined treatment group was perhaps related to the block of cell cycle in G2/M phase.

Key words: K562, apoptosis, Bcl-2/Bax, cell cycle