Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (2): 102-104.doi: 10.3969/j.issn.1004-616x.2009.02.006

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Inhibitory Effects of c9,t11-con-jugated Linoleic Acid on Matrix Metalloproteinase of SGC-7901 Cell Line via COX-2 Pathway

ZHANG Jing-shu1,CHEN Bing-qing2,YANG Yan-mei3, SUN Wen-guang2   

  1. 1.Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin 300011；2.Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin 150086；3. The Tumor Hospital for Harbin Medical University, Harbin 150081,China
  • Received:2008-06-19 Revised:2008-09-10 Online:2009-03-30 Published:2009-03-30

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Abstract: BACKGROUND AND AIM: To study the inhibitory effects of c9,t11-conjugated linoleic acid (c9,t11-CLA) on matrix metalloproteinase of human gastric carcinoma cell line(SGC-7901) via cyclooxygenase-2 pathway. MATERIALS AND METHODS: Inhibiting COX-2 activity by 100 μmol/L COX-2 inhibitor NS-398 in SGC-7901 cells, which were then treated with c9,t11-CLA by 200,100,50,25 μmol/L for 24 h. The expressions of MMP-2,MMP-9 mRNA were measured by RT-PCR. RESULTS: At the concentrations of 200,100,50,25 μmol/L, c9,t11-CLA decreased the expressions of MMP-2,MMP-9 mRNA. But when the SGC-7901 cells were treated with NS-398 in advance, MMP-2 and MMP-9 mRNA level were not decreased in any concentration of c9, t11-CLA groups. CONCLUSION: c9,t11-CLA could inhibit the expressions of MMP-2,MMP-9 mRNA in SGC-7901 cells, and COX-2 might play an important role in mediating the ability of c9,t11-CLA to inhibit the matrix metalloproteinase of tumor cells.

Key words: conjugated linoleic acid, cyclooxygenase-2, matrix metalloproteinase