Abstract: BACKGROUND AND AIM: To investigate the relationship between expression of CDC6 and TFF2 and HPV16/18 infection in uterine cervical cancer. MATERIALS AND METHODS: Formalin-fixed, paraffin-embedded samples including 50 cases of squamous cell carcinomas, 20 cervical intraepithelial neoplasia (CIN)Ⅱ-Ⅲ and 20 CINⅠ and 20 samples of normal cervical tissues were studied. Using Polymer DetectionSystem For Immuno-Histological Staining(PV-9000) immunohistochemical technique, we examined the expression of CDC6 and TFF2 in these samples. The infections of HPV type 16, 18 DNA were determined by PCR. RESULTS: There were significant differences for CDC6 among carcinomas, CIN and normal cervices(P<0.05). Expression of CDC6 was correlated with tumor grades and lymph node metastasis (all P<0.05). The positive expression of TFF2 in carcinomas was significantly lower than in normal cervical tissue and CIN (P<0.05). The positive rate of TFF2 was associated with age group (P<0.05)but not with tumor grades, clinical stages and lymph node metastasis(all P>0.05).The positive rate of HPV16/18 was significantly different between cervical carcinomas, CIN and normal tissues (P<0.05) but not between tumor grades, clinical stages, age group and lymph node metastasis (all P>0.05). The expression of CDC6 showed positive correlation with that of HPV16/18 (rs=0.386,P<0.05). HPV16/18 and CDC6 both showed negative correlations with TFF2 (rs=-0.500, P< 0.05;rs=-0.356,P<0.05,respectively). CONCLUSION: In tissues of CIN and cervical carcinomas, changes in CDC6 and TFF2 expression may be associated with HPV16/18 infection. Expressions of CDC6 was associated with the malignant level of uterine cervical cancer.

Key words: CDC6, TFF2, human papilloma virus, cervical cancer