Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (1): 44-049.doi: 10.3969/j.issn.1004-616x.2008.01.011

Previous Articles     Next Articles

Cigarette Smoke Condense_ induced Alteration of Transcriptional Profile in Human Bronchial Epithelial Cells

HU Ying_chun1, HUO Yan_ying1,, ZHONG Ke_jun2, YANG Zhi_hua1, XU Guo_rui1, PAN Xiu_jie1, WU De_chang1, ZHU Mao_xiang1   

  1. (1.Department of Radiation Toxicology and Oncology, Beijing Institute f Radiation Medicine, Beijing 100850;2.Center of Science ＆ echnology, Hunan Tobacco Company, Changde 415000, Hunan，China)
  • Received:2007-11-19 Revised:2007-11-19 Online:2008-01-30 Published:2008-01-30
  • Contact: Huo Yan_ying

PDF (PC)

2812

Abstract: BACKGROUND AND AIM: To explore the cigarette smoke condense(CSC) induced alteration of transcriptional profiling in papillomavirus_immortalized human bronchial epithelial cells(BEP2D). MATERIALS AND METHODS: We used CSC to treat BEP2D cells and examined the oncogenic transforming effects of CSC. Anchorage_ independent growth and the chromosome changes were used to investigate the various stages of transformation in BEP2D cells. Microarray assay was used to explore the CSC_induced alteration of transcriptional profiling. RESULTS: After CSC treatment, the P30 of BEP2D cells gained anchorage independence growth ability. The chromosomal number of the P40 cells was poly_ploid. In the earlier period of CSC treatment, 269 genes were down_regulated and 63 genes were up_regulated. In the advanced stage of CSC treatment, 316 genes were down_regulated and 147 genes were up_regulated. CONCLUSION: We successfully analyzed the alteration of transcriptional profile in BEP2D cells induced by CSC and identified a batch of genes related with the initiation and development of lung cancer.

Key words: cigarette smoke condense, bronchial epithelial cells, malignant transformation, transcriptional profile

CLC Number: