环磷酰胺诱发的染色体损伤与P53基因表达的关系

doi:10.3969/j.issn.1004-616x.2003.04.013

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2003, Vol. 15 ›› Issue (4): 228-230.doi: 10.3969/j.issn.1004-616x.2003.04.013

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RELATIONSHIP BETWEEN CHROMOSOME DAMAGE INDUCED BY CYCLOPHOSPHAMIDE AND EXPRESSION OF MUTATED P53 GENE

WANG Ming-yan1, XU Dong-qing1,XU Li 2,et al

1.Biological Teaching and Researching Section, Basic Medical College of Nanjing University of Traditional Chinese Medicine,2.The Editorial Department of Journal of Nanjing University of TCM, Nanjing 210029,China

Received:2002-07-17 Revised:2003-03-12 Online:2003-10-30 Published:2003-10-30
Contact: WANG Ming-yan

Abstract

Abstract: Purpose: To study the relation between chromosome damage induced by cyclophosphamide(CP) and the expression of mutated P53 gene. Methods: The expression of mutated P53 gene in human lymphocytes was investigated using flow cytometry.And the micronucleus(MN) and sister chromatid exchange (SCE) tests were studied in vitro. Results: The expression ofmutated P53 gene in human lymphocytes treated with CP was 30.81 ％, (P < 0.01)compared with the control .The frequencies of SCE and MN in human lymphocytes exposed with CP were 9.21±1.08 and 8 ‰ respectively(P < 0.01),compared with the control. Conclusion: The mutagenic effect of CP may be related to the expression of mutated P53 gene .

Key words: cyclophosphamide, mutagenicity, mutated P53 gene, micronucleus, sister chromatid exchange

WANG Ming-yan, XU Dong-qing, XU Li , et al. RELATIONSHIP BETWEEN CHROMOSOME DAMAGE INDUCED BY CYCLOPHOSPHAMIDE AND EXPRESSION OF MUTATED P53 GENE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2003, 15(4): 228-230.

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RELATIONSHIP BETWEEN CHROMOSOME DAMAGE INDUCED BY CYCLOPHOSPHAMIDE AND EXPRESSION OF MUTATED P53 GENE

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