Carcinogenesis, Teratogenesis & Mutagenesis ›› 2002, Vol. 14 ›› Issue (1): 10-012.doi: 10.3969/j.issn.1004-616x.2002.01.003
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LOU Tie-zhu; GE Shi-li; XIANG Xiao-qiong; WU De-chang
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Abstract: Purpose and Methods : To detect related genes mutation in human bronchial epithelial (BEP2D) cells transformed by α-particles and by using PCR amplification and single2strand conformation polymorphism ( SSCP) . Results : The point mutation of double-strand break repair gene XRCC5 occurred at the early stage of transformation , but no mutation was found in the exon-of p16 gene and exon12 of hMSH2 gene. Conclusion :Mutated XRCC5 gene can't repair DNA lesions induced byα-particles , and plays as one initiator in the process of transformation induced byα-particles.
Key words: alpha-particle, BEP2D cell, cell transformation, PCR-SSCP, XRCC5 gene
CLC Number:
R73412
R7301231
LOU Tie-zhu, GE Shi-li, XIANG Xiao-qiong, WU De-chang. DETECTING RELATED GENES MUTATION IN HUMAN BRONCHIAL EPITHELIAL CELL LINE BEP2D[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2002, 14(1): 10-012.
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