不同种类细胞株对UVB紫外线的耐受性初探

doi:10.3969/j.issn.1004-616x.2014.05.008

癌变·畸变·突变

不同种类细胞株对UVB紫外线的耐受性初探

高玲¹，李峰生²，封江彬¹，李翔文³，刘建香^1，*，刘青杰^1，*

中国疾病预防控制中心辐射防护与核医学安全所，辐射防护与核应急中国疾病预防控制中心重点实验室，北京 100088

收稿日期:2014-05-19 修回日期:2014-09-12 出版日期:2014-09-30 发布日期:2014-09-30
通讯作者: 刘建香，E-mail：jxliu@nirp.cn；刘青杰，E-mail：qjliu@nirp.cn
作者简介:高玲(1978- )，女，新疆奎屯人，副研究员，研究方向：辐射损伤分子机制。E-mail：gaoling@nirp.cn
基金资助:
国家自然科学基金(31340051，81202151，81001216)

Investigation of the tolerance of various kinds of cell lines to ultraviolet B radiation

GAO Ling¹，LI Feng-sheng²，FENG Jiang-bin¹，LI Xiang-wen³，LIU Jian-xiang^1，*，LIU Qing-jie^1，*

National Institute for Radiological Protection, Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Beijing 100088

Received:2014-05-19 Revised:2014-09-12 Online:2014-09-30 Published:2014-09-30

摘要/Abstract

摘要：

目的：探索几种细胞株对UVB紫外线的耐受性。方法：0、20和50 mJ/cm2紫外线照射后，利用MTT、荧光素酶报告基因检测和克隆形成率实验，检测人表皮细胞HaCaT、人黑色素细胞A875、人肺腺癌细胞A549和H322、人肝癌细胞HepG2的存活及增殖能力。结果：MTT实验结果表明HaCaT、A875、A549、H322和HepG2细胞在受到20和50 mJ/cm2紫外线照射后第1天，细胞活力均显著降低(P<0.05)，到照射后第3天，细胞活力仍显著低于未照射组(P<0.05)。克隆形成率实验结果表明，HaCaT、A875和HepG2细胞在照射后，细胞增殖能力受到显著抑制。荧光素酶活性检测实验结果表明，A549细胞在受到20和50 mJ/cm2紫外线照射后第5和第10天，细胞活力显著低于未照射组(P<0.05)。结论：UVB紫外线对表皮细胞、肺腺癌细胞和肝癌细胞的存活和增殖能力均产生影响，其中正常表皮细胞HaCaT细胞对UVB紫外线的耐受性大于肿瘤来源的A875、A549、H322和HepG2细胞。

关键词: UVB紫外线, 细胞存活, 增殖, 耐受性

Abstract:

OBJECTIVE: To investigate the tolerance of various kinds of cell lines to ultraviolet B radiation. METHODS：Cell viability and proliferation were detected by MTT，firefly luciferase activity and cloning efficiency assays after cells including epidermal cells (HaCaT)，melanocytes (A875)，pulmonary adenocarcinoma cells (A549 and H322) and hepatocarcinoma cells (HepG2) of human were irradiated with ultraviolet B at dose of 20 or 50 mJ/cm2. RESULTS：Cell viability including epidermal cells (HaCaT)，melanocytes (A875)，pulmonary adenocarcinoma cells (A549 and H322) and human hepatocarcinoma cells (HepG2)，decreased significantly 1 day after ultraviolet B irradiation at doses of 20 and 50 mJ/cm 2 (P<0.05). The viability of these cells were still lower than untreated control cells 3 days after irradiation. Finally，the proliferative ability of all the examined cells was suppressed after ultraviolet B irradiation at doses of 20 and 50 mJ/cm2(P<0.05)，as shown by cloning efficiency assays. Further firefly luciferase activity assay indicated that the viability of A549 cells were suppressed 5 and 10 days after exposure(P<0.05). CONCLUSION：Ultraviolet B irradiation affected the viability and proliferation of epidermal cell，lung adenocarcinma cell and hepatoma cell. Normal epidermal cell HaCaT possessed the more tolerance than A875，A549，H322 and HepG2 cells which is the source of tumors on ultraviolet B.

Key words: UVB ultraviolet, cell viability, proliferation, tolerance

高玲，李峰生，封江彬，李翔文，刘建香，刘青杰. 不同种类细胞株对UVB紫外线的耐受性初探[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2014.05.008.

GAO Ling，LI Feng-sheng，FENG Jiang-bin，LI Xiang-wen，LIU Jian-xiang，LIU Qing-jie. Investigation of the tolerance of various kinds of cell lines to ultraviolet B radiation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.05.008.

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不同种类细胞株对UVB紫外线的耐受性初探

Investigation of the tolerance of various kinds of cell lines to ultraviolet B radiation

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